ANALYSIS OF VIABLE AND NONVIABLE CELLS

Provided are methods, compositions, and kits for selectively analyzing a sample for viable cells and nonviable cells. For example, the method may include dyeing viable cells and/or nonviable with a membrane-permeable fluorescent dye, and dyeing nonviable cells with a membrane-impermeable fluorescent...

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Bibliographic Details
Main Authors Apfel, Christian, Moroz, Ekaterina, Anderson, Kraig K
Format Patent
LanguageEnglish
Published 16.04.2020
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:Provided are methods, compositions, and kits for selectively analyzing a sample for viable cells and nonviable cells. For example, the method may include dyeing viable cells and/or nonviable with a membrane-permeable fluorescent dye, and dyeing nonviable cells with a membrane-impermeable fluorescent quenching dye. The method may include illuminating to cause fluorescent emission from the membrane-permeable fluorescent dye in the viable cells and the membrane-impermeable fluorescent quenching dye in the non-viable cells. The method may include quenching at least a portion of fluorescence of the membrane-permeable fluorescent dye in the nonviable cells by the membrane-impermeable fluorescent quenching dye. The method may include selectively analyzing the sample for the viable and nonviable cells.
Bibliography:Application Number: US201916600339