A Method and System for Separating Biomolecules

A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: -providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a...

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Bibliographic Details
Main Authors Palmgren, Ronnie, Norrman, Nils, Lundin, Andreas Torbjorn, Lind, Ola, Avallin, Johan Fredrik, Rodrigo, Gustav Jose
Format Patent
LanguageEnglish
Published 19.03.2020
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: -providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; -separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; -forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); -eluting the bound biomolecules in the elution cell.
Bibliography:Application Number: US201716470607