PREVENTION OF PROTEIN DISULFIDE BOND REDUCTION

Disclosed are methods for testing the presence and/or activity of glutathione system components and thioredoxin system components during the manufacturing process of disulfide bond-containing proteins. Also disclosed are methods for mitigating reduction of disulfide bonds during the manufacturing pr...

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Main Authors HANDLOGTEN, Michael, CHUNG, Wai Keen, ZHU, Min, HWANG, Kenneth, WANG, Jihong, NARANG, Someet, RUSSELL, Brian, HUDAK, Suzanne, GOLDBERG, Deborah Sweet, AHUJA, Sanjeev
Format Patent
LanguageEnglish
Published 18.04.2019
Subjects
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Summary:Disclosed are methods for testing the presence and/or activity of glutathione system components and thioredoxin system components during the manufacturing process of disulfide bond-containing proteins. Also disclosed are methods for mitigating reduction of disulfide bonds during the manufacturing process, and for lowering the reduction potential of disulfide bond-containing proteins. Provided are compositions, kits, and methods for mitigating reduction and diminishing reduction potential of disulfide bond-containing proteins during protein manufacturing processes.
Bibliography:Application Number: US201716300315