MICROSCOPE AND METHOD FOR PRODUCING A MICROSCOPIC IMAGE WITH EXPANDED DEPTH OF FIELD

• Main Authors: GAIDUK, Alexander, KUMAR, Rohith, ILIOPOULOS, Pavlos, KALININ, Stanislav
• Format: Patent
• Language: English
• Published: 11.04.2019
• Subjects: OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHYSICS

The present invention relates firstly to a method for producing a microscopic image with an extended depth of field by means of a microscope. In a step of the method, a plurality of microscopic frames of a specimen are acquired from different focus positions, for example with different dimensions of a distance between the specimen and an objective lens of the microscope. In another step, the plurality of microscopic frames are processed so as to form a microscopic image with an extended depth of field. According to the invention, the focus position is continuously changed during the acquisition of at least some of the microscopic frames at a variable speed or with variable acceleration. The invention further relates to a microscope with an objective lens for optically imaging a specimen.