A Stain for Staining Cells, the Use of the Stain in Histological Applications and a Method of Diagnosis

A staining composition for staining cells and for use in a method of staining cells, a method of diagnosing mitotic activity and a method of diagnosing cancer. The composition comprises dried petunia petals at least partially dissolved in distilled water; and 400 milligrams of picric acid per gram o...

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Bibliographic Details
Main Author Alshamar, Hayfaa Abd Ali Mohammed
Format Patent
LanguageEnglish
Published 06.09.2018
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:A staining composition for staining cells and for use in a method of staining cells, a method of diagnosing mitotic activity and a method of diagnosing cancer. The composition comprises dried petunia petals at least partially dissolved in distilled water; and 400 milligrams of picric acid per gram of dried petunia petals. The method of staining cells includes rinsing a sample containing the cells with 10% of phosphotungistic acid or 10% of phosphomolybdic acid; staining the sample with the composition and dehydrating the samples with 100% Ethanol or Isopropanol. The method of diagnosing mitotic activity includes observing stained regions in the sample to identify mitotic activity indicated by stained regions. The method for diagnosing cancer includes quantifying mitotic figures in the sample which are identified by stained regions of the sample.
Bibliography:Application Number: US201815913205