DEVICES AND METHODS FOR SAMPLE ANALYSIS

• Main Authors: Leary, Thomas, Pope, Mark R, Tetin, Sergey, Holets-McCormack, Shelley R, Hershberger, Stefan, Qiao, Lei, Murphy, M. Shawn, Ruan, QiaoQiao, House, Dustin, Huff, Jeffrey B, Haack, Richard, Prieto-Ballengee, Edna M, Yarnell, Lyle, Karabatsos, Peter J, Fischer, Andrew T, Bogdan, Felicia, Soni, Pathik, Hayden, Mark A, Muerhoff, Anthony S, Schapals, Andrew S, Laurenson, Sophie
• Format: Patent
• Language: English
• Published: 05.07.2018
• Subjects: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; TESTING; TRANSPORTING

Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.