High Speed Nucleic Acid Melting Analysis

A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing....

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Bibliographic Details
Main Authors Pryor, Robert J, Sundberg, Scott O, Knight, Ivor T, Wittwer, Carl T, Paek, Jeanette Y, Myrick, Joseph T, Palais, Robert A
Format Patent
LanguageEnglish
Published 26.04.2018
Subjects
BEER
BIOCHEMISTRY
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TRANSPORTING
VINEGAR
WINE
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Summary:A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50° C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons.
Bibliography:Application Number: US201715664843