METHOD OF DOUBLE ALLELE SPECIFIC PCR FOR SNP MICROARRAY
The present invention provides a method of double allele specific polymerase chain reaction (PCR) for single nucleotide polymorphism (SNP) microarray, the method provides the allele specific site as the 3′ teiiiiinal nucleotide of forward and reverse primers, it does not require a primer having spec...
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Main Author | |
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Format | Patent |
Language | English |
Published |
29.06.2017
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention provides a method of double allele specific polymerase chain reaction (PCR) for single nucleotide polymorphism (SNP) microarray, the method provides the allele specific site as the 3′ teiiiiinal nucleotide of forward and reverse primers, it does not require a primer having specific nucleotides. The method of the present invention is easily to design the primer based on flanking region of the allele specific site, and to perform multiplex SNP PCR applying with an interchelating agent, then to detect by a SNP microarray. |
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Bibliography: | Application Number: US201615162907 |