METHOD OF DOUBLE ALLELE SPECIFIC PCR FOR SNP MICROARRAY

The present invention provides a method of double allele specific polymerase chain reaction (PCR) for single nucleotide polymorphism (SNP) microarray, the method provides the allele specific site as the 3′ teiiiiinal nucleotide of forward and reverse primers, it does not require a primer having spec...

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Bibliographic Details
Main Author LIN Shang-Chi
Format Patent
LanguageEnglish
Published 29.06.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides a method of double allele specific polymerase chain reaction (PCR) for single nucleotide polymorphism (SNP) microarray, the method provides the allele specific site as the 3′ teiiiiinal nucleotide of forward and reverse primers, it does not require a primer having specific nucleotides. The method of the present invention is easily to design the primer based on flanking region of the allele specific site, and to perform multiplex SNP PCR applying with an interchelating agent, then to detect by a SNP microarray.
Bibliography:Application Number: US201615162907