Mesenchymal Stem Cells Derived From Induced Pluripotent Stem Cells

A method of producing mesenchymal stem cells from induced pluripotent stem cells in which induced pluripotent stem cells are cultured in the presence of a TGF-βinhibitor an in an atmosphere containing from about 7 vol. % to about 8 vol. % C02 for a period of time from about 20 day to about 35 days....

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Bibliographic Details
Main Authors Zhao Qingguo, Berkowitz Barry A, Prockop Darwin J, Liu Fei
Format Patent
LanguageEnglish
Published 25.05.2017
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method of producing mesenchymal stem cells from induced pluripotent stem cells in which induced pluripotent stem cells are cultured in the presence of a TGF-βinhibitor an in an atmosphere containing from about 7 vol. % to about 8 vol. % C02 for a period of time from about 20 day to about 35 days. The cells then are transfered to a culture dish having a hydrophilic sur face, and the cells are cultured in a medium containing a TGF-β inhibitor for a period of time sufficient to produce mesenchymal stem cells. Such mesenchymal stem cells are more stable and less likely to form tumors, cancers, or teratomas. Also, the induced pluripotent stem cells may be genetically engineered with at least one polynucleotide encoding a therapeutic agent and then are cultured as hereinabove described to provide genetically engineered mesenchymal stem cells that express sustained amounts of a biologically active protein or polypeptide.
Bibliography:Application Number: US201515323893