HEPARIN AFFINITY TAG AND APPLICATIONS THEREOF

In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant pr...

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Bibliographic Details
Main Authors Morris Jacqueline, Brown Alicia, McNabb David, Jayanthi Srinivas, Thallapuranam Suresh Kumar, Henry Ralph
Format Patent
LanguageEnglish
Published 02.02.2017
Subjects
BEER
BIOCHEMISTRY
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SEPARATION
SPIRITS
THEIR RELEVANT APPARATUS
TRANSPORTING
VINEGAR
WINE
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Summary:In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine.
Bibliography:Application Number: US201515112984