Multiplexed Olfactory Receptor-Based Microsurface Plasmon Polariton Detector

• Main Authors: BIKKER JOHANNA F, OHNISHI DAI, ZHANG SHUGUANG, NIWA DAISUKE, MIURA YOSHIKATSU, MERSHIN ANDREAS, COOK BRIAN, KAISER LISELOTTE, TAZUKE ATSUSHI
• Format: Patent
• Language: English
• Published: 11.12.2014
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a bio-sensing nanodevice comprising: a stabilized G-protein coupled receptor on a support, a real time receptor-ligand binding detection method, a test composition delivery system and a test composition recognition program. The G-protein coupled receptor can be stabilized using surfactant peptide. The nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized G-protein coupled receptor immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.