USE OF FMN-BINDING FLUORESCENCE PROTEINS (FBFP) AS NEW TYPES OF SECRETION MARKERS

• Main Authors: DREPPER THOMAS, JAEGER KARL-ERICH, POTZKEI JANKO
• Format: Patent
• Language: English
• Published: 14.08.2014
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

To investigate protein-protein interactions, protein foldings and protein localization and also in the secretion of proteins, in vivo reporter proteins are used in biotechnology and in basic research. In order to be able to utilize fluorescence reporters as markers for secretion processes, FMN-binding fluorescence proteins (FbFP) have been developed by us for the first time. The new fluorescence markers can be expressed like GFP in various bacteria. The binding of the chromophore FMN produces a cyan-green fluorescent protein which can be detected in vivo using all customary spectroscopic and microscopic methods. In contrast to GFP, this protein can also surprisingly be secreted via the Sec route and be converted to the fluorescence-active state in the periplasma.