METHOD FOR TARGET PROTEIN NORMALIZATION

The present invention relates to a method for target protein normalization, especially for Western blotting applications. More closely, the invention relates to a method for normalizing target protein signals, after electrophoresis and Western blotting, against variations of sample load or cell numb...

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Bibliographic Details
Main Authors RONN OLA, HAGNER-MCWHIRTER ASA
Format Patent
LanguageEnglish
Published 17.04.2014
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:The present invention relates to a method for target protein normalization, especially for Western blotting applications. More closely, the invention relates to a method for normalizing target protein signals, after electrophoresis and Western blotting, against variations of sample load or cell number between different lanes or within the same lane on an electrophoretic gel. The signals are normalized against the total protein signal (=ratio between target protein/total protein) or reference protein band signal(s) (=ratio between target protein/reference protein band). According to the invention multiplex and quantitative assessments are possible, such as quantitative comparison between target proteins in different samples.
Bibliography:Application Number: US201214122382