METHOD FOR DETECTING THE PRESENCE OF A NUCLEIC ACID IN A SAMPLE
An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette o...
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Main Authors | , , , , , , , , |
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Format | Patent |
Language | English |
Published |
09.01.2014
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Subjects | |
Online Access | Get full text |
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Summary: | An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette of the analyzer is used to form a reaction mixture comprising the purified nucleic acid and all reagents required to perform a nucleic acid amplification. As a result of the nucleic acid amplification, amplification products are synthesized that include a nucleotide sequence contained in the nucleic acid or the complement of the nucleic acid. The amplification products are exposed to a probe in a mixture, where the probe forms a hybrid with one of the amplification products. A label associated with the probe is detected as an indication of the formation of the hybrid, and the formation of the hybrid in the mixture provides an indication of the presence of the nucleic acid in the sample. |
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Bibliography: | Application Number: US201314024261 |