SCREENING METHODS OF MODULATING ADRENERGIC RECEPTOR GENE EXPRESSIONS IMPLICATED IN MELANOGENESIS

• Main Authors: BIERMAN JOHN CRIST, LAUGHLIN, II LEO TIMOTHY, ZHAO WENZHU, WANG JIAZHEN, HAKOZAKI TOMOHIRO
• Format: Patent
• Language: English
• Published: 02.08.2012
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

Embodiments of screening methods for determining test agents effective for modifying the appearance of pigmented skin are provided. The screening method may comprise the steps of contacting a cell, a cell culture, or bulk cells with the test agent, wherein the cell, the cell culture, or the bulk cells comprise ADR 1 receptors, and determining based on the binding interaction of the test agent with the ADR 1 receptors whether the test agent is an effective ADR 1 receptor antagonist suitable for modifying the appearance of pigmented skin, wherein a test agent is considered to be an effective ADR 1 receptor antagonist when it defines a half maximal inhibitory concentration of less than about 1000 ppm.