RECOMBINANT DNA MOLECULE ENCODING 5' UTR CAPABLE OF PREVENTING INHIBITION OF TRANSLATION UNDER ENVIRONMENTAL STRESSES

• Main Authors: MATSUURA HIDEYUKI, KANAYA SHIGEHIKO, KUBO YUHKI, NOSHO TATSUYA, UEDA KIYOTAKA, TAKENAMI SHINYA, KATO KO
• Format: Patent
• Language: English
• Published: 05.07.2012
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CALCULATING; CHEMISTRY; COMPOSITIONS THEREOF; COMPUTING; COUNTING; CULTURE MEDIA; DERIVATIVES THEREOF; ELECTRIC DIGITAL DATA PROCESSING; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PHYSICS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; TRAPPING; VINEGAR; WINE

An object of the present invention is to identify sequence features of the 5′ UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5′ UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector: (a) 5′ UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5′ end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and (b) 5′ UTR in which one or more bases in the base sequence of the 5′ UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.