TARGET DNA DETECTION METHOD AND TARGET DNA DETECTION KIT

• Main Author: TANABE TETSUYA
• Format: Patent
• Language: English
• Published: 19.05.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention provides a method for detecting a target DNA easily and highly accurately through simultaneous analysis of a sense strand and an antisense strand of the target DNA, and a kit therefor. The target DNA detection method of the present invention is a method for detecting a target DNA composed of a sense strand having a target nucleotide sequence and an antisense strand complementary to the sense strand, wherein: a first oligonucleotide which hybridizes with the sense strand, and a second oligonucleotide which hybridizes with the antisense strand are used; at least a part of a region, of the antisense strand, which hybridizes with the second oligonucleotide is complementary to at least a part of a region, of the sense strand, which hybridizes with the first oligonucleotide; and the first oligonucleotide and the second oligonucleotide are simultaneously added into one reaction solution to effect respective hybridizations with the target DNA, followed by ligation reactions and/or PCR, thereby detecting resultant products.