Novel Polymer and Method of Measuring Cholesterol Therewith

• Main Authors: YAMAMOTO NAOYUKI, ITAI TOMOKAZU, YAGURA YO, KOYAMA ISAO, MORI KIYOKO
• Format: Patent
• Language: English
• Published: 27.08.2009
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVINGCARBON-TO-CARBON UNSATURATED BONDS; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP; VINEGAR; WINE

It is an object of the present invention to provide the method for determining cholesterol in lipoprotein and reagent for determining cholesterol comprising the polymer, and the present invention relates to the reagent for determining cholesterol in lipoproteins such as high-density lipoprotein (HDL), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL) and the determination method using the polymer comprising the following units as constituents: (iv) a polyethylene glycol segment represented by the general formula [1]: -(CH2CH2O)k- [1] (wherein, k represents an integer of 10 to 250); (v) a monomer unit represented by the general formula [2]: (wherein R1 represents a hydrogen atom or C1-C3 alkyl group); and (vi) a monomer unit represented by the general formula [3]: [wherein, R2 represents a hydrogen atom or C1-C3 alkyl group, and R3 represents a group represented by the general formula [4]: -COOR4 [4] (wherein, R4 represents an alkyl group, a haloalkyl group or a bornyl group), an alkyl group, an alkoxy group, an aralkyl group or an alkylcarbamoyl group; particularly, in the direct measurement of cholesterols such as HDL, LDL and VLDL contained in the sample, the reagent for determining cholesterol in the presence of said polymer, and the method for determining the concentration of cholesterol in a specific lipoprotein using said reagent for determining cholesterol, and a novel polymer.