USE OF DENATURING AGENTS DURING AFFINITY CAPTURE

A method for affinity capturing target molecules is disclosed, comprising obtaining a sample comprising a target molecule; and, in the presence of a denaturing agent; affinity capturing the target molecule with a single chain affinity molecule or antigen binding portion thereof that specifically bin...

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Bibliographic Details
Main Author BRADBURY LAURA
Format Patent
LanguageEnglish
Published 20.08.2009
Subjects
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
TRANSPORTING
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Summary:A method for affinity capturing target molecules is disclosed, comprising obtaining a sample comprising a target molecule; and, in the presence of a denaturing agent; affinity capturing the target molecule with a single chain affinity molecule or antigen binding portion thereof that specifically binds the target molecule, wherein the affinity molecule or antigen binding portion thereof is immobilized on a support.
Bibliography:Application Number: US20070307596