Potent inhibition of influenza virus by specifically designed short interfering RNA

• Main Authors: TRAN LYNN M, PAL BIJAY K
• Format: Patent
• Language: English
• Published: 28.06.2007
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

This patent application discloses siRNA sequences against the constant region of the influenza virus nucleoprotein gene comprising: Sense strand: 5' UGAAGGAUCUUAUUUCUUCdTdT 3' Anti sense strand: 3' dTdTACUUCCUAGAAUAAAGAAG 5' or Sense strand: 5' UGAAGGAUCUUAUUUCUUCGGdTdT 3' Anti sense strand: 3' dTdTACUUCCUAGAAUAAAGAAGCC 5' or Sense strand: 5' GGAUCUUAUUUCUUCGGAGACdTdT 3' Anti sense strand: 3' dTdTCCUAGAAUAAAGAAGCCUCUG 5' said sequences being inhibitory against influenza virus in animals including humans. The invention further includes one or more of said siRNA sequences in the form of an aqueous suspension suitable for nasal inhalation. Still further, the invention includes one or more of said siRNA sequences in the form of a plasmid expressing intracellularly in animals including humans. In another aspect, the invention includes one or more of said siRNA sequences in the form of an AAV vector adapted to express intercellularly and establish a permanent inhibitory effect against influenza virus by integrating to the cellular chromosome of animals including humans. The invention also includes the administration to an animal including humans, in a therapeutically effective amount, at least one of said siRNA sequences.