Method and array for detection and identification of microorangisms present in a sample using the genomic regions coding for different tRNA synthetases

• Main Authors: ARAVENA DUARTE ANDRES O, MORENO CORTEZ PABLO ANDRES, PARADA VALDECANTOS PILAR A
• Format: Patent
• Language: English
• Published: 14.06.2007
• Subjects: BEER; BIOCHEMISTRY; CALCULATING; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPUTING; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; COUNTING; ELECTRIC DIGITAL DATA PROCESSING; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention discloses a method to detect and identify microorganisms that are present in a sample, which comprises hybridizing total DNA isolated from the sample with DNA fragments from regions that codifies for tRNA-synthases, hereinafter 'tRNA-synthases', said fragments being selected due to their specificity for each taxon to be detected and identified. Furthermore, an array for detection and identification of microorganisms is disclosed, which comprises at least one tRNA-synthase fragment bound to its surface that is specific for each taxon to be detected and identified and has been designed according to the method described in the present document.