Structure-based designed herbicide resistant products

• Main Authors: STOCKTON GERALD W, KWAGH JAE-GYU, KAKEFUDA GENICHI, OTT KARL-HEINZ
• Format: Patent
• Language: English
• Published: 13.07.2006
• Subjects: AGRICULTURE; ANALOGUE COMPUTERS; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CALCULATING; CHEMISTRY; COMPOSITIONS THEREOF; COMPUTING; COUNTING; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

Disclosed herein are structure-based modelling methods for the preparation of acetohydroxy acid synthase (AHAS) variants, including those that exhibit selectively increased resistance to herbicides such as imidazoline herbicides and AHAS inhibiting herbicides. The invention encompasses isolated DNAs encoding such variants, vectors that include the DNAs, and methods for producing the variant polypeptides and herbicide resistant plants containing specific AHAS gene mutations. Methods for weed control in crops are also provided.