Screening method

• Main Authors: MARKS BRUNO, FLETCHER LAURA, TAVARE JEREMY
• Format: Patent
• Language: English
• Published: 16.06.2005
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

There is disclosed an assay to determine the effect of an applied agent or influence or other stimulus on the sub-cellular localisation of the expressed product of a nucleotide sequence ("the nucleotide sequence under investigation"), comprising: (i) transfecting a cell population with a cloned expression vector comprising a composite nucleotide sequence encoding the nucleotide sequence under investigation and a detectable tag, arranged such that the product of the expressed composite nucleotide sequence includes the expressed detectable tag; (ii) applying the applied agent, influence or other stimulus to at least a portion of the transfected cell population; (iii) observing the sub-cellular location of the detectable tag in the transfected cell population and comparing the observed sub-cellular localisation to the sub-cellular localisation of the detectable tag in the cell population in the absence of said applied agent or influence.