Method of analysis of aldehyde and ketone by mass spectrometry

• Main Authors: NGUYEN DUC TIEN, NGUYEN TRINH DUC, NGUYEN HOA DUC
• Format: Patent
• Language: English
• Published: 31.03.2005
• Edition: 7
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Method of identification and quantitative analysis of aldehyde(s) and/or ketone(s) in a sample by mass spectrometry using stable isotope labeled internal standard is provided. Said internal standard is prepared by reaction of an authentic sample of said aldehyde(s) and/or ketone(s) with a stable isotope labeled reagent, and is added to a sample containing said aldehyde(s) and/or ketone(s). Said aldehyde(s) and/or ketone(s) in said sample is then quantitatively converted to a chemical compound of identical structure, except the stable isotope atoms, as that of said internal standard using a non-labeled reagent. Said sample is then extracted and the extract is analyzed by mass spectrometry. Identification and quantification of said aldehyde(s) and/or ketone(s) are made from a plot of ion ratio of said converted aldehyde and/or ketone to said internal standard versus aldehyde and/or ketone concentration.