Oligonucleotide, method and system for detecting antibiotic resistance-mediating genes in microorganisms by means of real-time PCR

An oligonucleotide, a method and a system for detecting antibiotic resistance-mediating genes in microorganisms by means of real-time PCR, comprising: the use of a first primer nucleotide sequence (A) which is selected from the group of sequences consisting of SEQ# 1-4, the use of a second primer nu...

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Bibliographic Details
Main Authors KIRCHEN SILKE, OBST URSULA, VOLKMANN HOLGER, SCHWARTZ THOMAS
Format Patent
LanguageEnglish
Published 17.03.2005
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:An oligonucleotide, a method and a system for detecting antibiotic resistance-mediating genes in microorganisms by means of real-time PCR, comprising: the use of a first primer nucleotide sequence (A) which is selected from the group of sequences consisting of SEQ# 1-4, the use of a second primer nucleotide sequence (B) which is selected from the group of sequences consisting of SEQ# 5-8, with the sequences 1 and 5, 2 and 6, 3 and 7, and 4 and 8 being used as primer pairs, and the use of at least one first dye (C) for detecting the PCR-amplified DNA, and their use, in particular on a biochip.
Bibliography:Application Number: US20040928647