Assay and kit for homocysteine

A method and kit is provided for assaying homocysteine in a biological sample containing homocysteine and cysteine. A competing compound with an amino group (-NH2) is mixed with the biological sample. An aldehyde, e.g. o-phthalaldehyde, is added to the biological sample to form homocysteine complex...

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Bibliographic Details
Main Authors SHIUE CHIAANN, SU MEI-FANG, CHANG JEN-HAO, WU TZU-I, LEE SU-JAN, WU CHIHUNG
Format Patent
LanguageEnglish
Published 27.01.2005
Edition7
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:A method and kit is provided for assaying homocysteine in a biological sample containing homocysteine and cysteine. A competing compound with an amino group (-NH2) is mixed with the biological sample. An aldehyde, e.g. o-phthalaldehyde, is added to the biological sample to form homocysteine complex with fluorescence. The concentration of homocysteine in the biological sample is determined according to the fluorescent intensity.
Bibliography:Application Number: US20030621371