Hybrid gene libraries and uses thereof

This invention relates to the construction and use of hybrid gene cDNA libraries. The vectors of such libraries each comprise a hybrid protein region in which cDNA is placed upstream of a sequence encoding a common peptide. The cDNA population inserted into the hybrid proteins is derived from an mRN...

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Bibliographic Details
Main Author EDWARDS DAVID N
Format Patent
LanguageEnglish
Published 16.09.2004
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:This invention relates to the construction and use of hybrid gene cDNA libraries. The vectors of such libraries each comprise a hybrid protein region in which cDNA is placed upstream of a sequence encoding a common peptide. The cDNA population inserted into the hybrid proteins is derived from an mRNA template population using random primers, thus providing better representation of the 5' end than if poly-T primers were used. The vector lacks a start codon before the multiple cloning site or in the common peptide so that only cDNA inserts containing a start codon result in a hybrid protein.
Bibliography:Application Number: US20040811026