Production of heterologous protein in a minimal culture medium

The process to produce heterologous proteins in a minimal culture medium consists of (i) transforming a bacterial strain that is secretary in a minimal culture medium with a plasmid that contains the coding sequence of said heterologous protein, (ii) culturing said strain in a minimal culture medium...

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Main Authors VARELA ESPINOSA JAVIER, RAMON GONZALEZ ALVARO, FERNANDEZ TORNERO CARLOS, GIMENEZ GALLEGO GUILLERMO, PIVEL RANIERI JUAN PABLO, ALONSO LEBRERO JOSE LUIS
Format Patent
LanguageEnglish
Published 22.07.2004
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The process to produce heterologous proteins in a minimal culture medium consists of (i) transforming a bacterial strain that is secretary in a minimal culture medium with a plasmid that contains the coding sequence of said heterologous protein, (ii) culturing said strain in a minimal culture medium, and (iii) recovering the heterologous protein. The bacterial strain that is secretary in a minimal culture medium can be obtained by means of a process that comprises (a) transforming bacteria adapted to grow in a minimal culture medium with a plasmid that comprises a DNA sequence that encodes for a protein that is toxic for a bacteria when it accumulates in the cytoplasm and whose synthesis is coupled to the secretion into the periplasm, (b) culturing said transformed bacteria in a minimal culture medium, and (c) selecting the surviving bacteria. It is applicable in the production of proteins of interest in bacteria that are secretary in a minimal culture medium.
Bibliography:Application Number: US20030346978