Compositions, methods and kits for allele discrimination

• Main Authors: HU XIUYUAN, WALKERPEACH CINDY R
• Format: Patent
• Language: English
• Published: 28.03.2002
• Edition: 7
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to sequence-specific polynucleotide probes, pairs of probes, the design of pairs of probes in relation to the strands of target nucleic acid, and coordinate sequence-specific pairs of polynucleotide primers, and also relates to kits containing such probes, probe pairs, primers, and primer pairs. The invention thus relates to kits and methods employing such polynucleotides. The invention relates to six specific allele discrimination kits. The specific kits are developed for the detection of four single base substitutions in the human CCR2, SDF1, Factor V, MTHFR, Factor XIII genes, and a 32-bp deletion in the human CCR5 gene. The capability of using two allele-specific molecular beacons in the same PCR solution enables the simultaneous determination of three possible allelic representations of a given sequence change in target DNA (Allele 1/Allele 1, Allele 2/Allele 2, Allele 1/Allele 2). It also definitively differentiates a true negative signal that is due to the absence of an allele from a false negative signal that results from PCR failure.