System and method with fiducials responding to multiple excitation frequencies

• Main Authors: Bowen, M. Shane, Nemiroski, Alex, Tanner, Stephen, Vieceli, John S, Chan, Danny Yuan, Samiee, Kevan, Belitz, Paul, Venkatesan, Bala Murali K, Langlois, Robert, Han, Hui
• Format: Patent
• Language: English
• Published: 13.02.2024
• Subjects: BEER; BIOCHEMISTRY; CALCULATING; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPUTING; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; COUNTING; ENZYMOLOGY; IMAGE DATA PROCESSING OR GENERATION, IN GENERAL; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; THEIR RELEVANT APPARATUS; TRANSPORTING; VINEGAR; WINE

Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include an "always on" type that respond to multiple frequencies of radiation used during processing and detection so as to return signals during successive cycles of imaging.