Dark tracking, hybrid method, conical diffraction microscopy, and dark addressing
A super resolution technique, intended mainly for fluorescence microscopy, acquires the three-dimensional position of an emitter, through a hybrid method, including a number of steps.In a first step the two-dimensional position of an emitter is acquired, using a technique, named in this application...
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Main Author | |
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Format | Patent |
Language | English |
Published |
31.01.2023
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Subjects | |
Online Access | Get full text |
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Summary: | A super resolution technique, intended mainly for fluorescence microscopy, acquires the three-dimensional position of an emitter, through a hybrid method, including a number of steps.In a first step the two-dimensional position of an emitter is acquired, using a technique, named in this application as an Abbe's loophole technique. In this technique a doughnut, or a combination of distributions, having a zero intensity at the combined center of the distributions, is projected onto the sample containing the emitter, under conditions wherein the doughnut null is moved towards the emitter to reach a position in which the emitter does not emit light.In a second step, an axial measurement is obtained using a 3D shaping method, characterized by the fact that the emitted light is shaped by an additional optical module creating a shape of the light emitted by the emitter, this shape being dependent of the axial position and means to retrieve the axial position from the shape. |
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Bibliography: | Application Number: US202017110018 |