Insect-resistant herbicide-tolerant corn transformation event

• Main Authors: She, Qiuming, Nie, Dongming, Han, Yu, Xu, Jieting, Tan, Chao, Tian, Peixiuzi, Liu, Bolin, Wang, Xuxia, Ma, Chonglie, Zhang, Wanggen
• Format: Patent
• Language: English
• Published: 25.10.2022
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

An insect-resistant herbicide-tolerant corn transformation event, and a related creation method, a detection method, and application thereof are provided herein. Using the corn inbred line Xiang 249 as a receptor, by means of agrobacterium-mediated genetic transformation, obtaining a corn plant with an exogenous gene insert inserted at a specific genomic locus, the exogenous gene insert comprising the following three genes: an insect-resistant gene, a glufosinate resistant gene, and a glyphosate resistant gene. In the obtained transformation event, the inserted exogenous genes are positioned at a non-functional locus of the corn genome, and do not affect the expression of the other genes of the receptor plant, such that the transgenic corn plant maintains good agronomic traits whilst acquiring insect resistance and herbicide tolerance.