Illumination for fluorescence imaging using objective lens

A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a...

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Bibliographic Details
Main Authors Bowen, M. Shane, Condello, Danilo, Krumbuegel, Marco A, Liu, Aaron, Obradovic, Bojan, Nemiroski, Alex, Pratt, Mark, Dehlinger, Dietrich, Erie, Frederick, Hong, Stanley S, Lam, Anthony, Sun, Yinghua
Format Patent
LanguageEnglish
Published 14.06.2022
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a first image sensor to capture imaging light using the objective lens, wherein the first grating is positioned outside a field of view of the first image sensor. Dual-surface imaging can be performed. Flowcells with multiple swaths bounded by gratings can be used. An auto-alignment process can be performed.
Bibliography:Application Number: US201916351193