Carrier for bio-related molecule immobilization

• Main Authors: Nakajima, Kazuki, Kashiwabara, Ken, Akuzawa, Norio, Saruwatari, Yoshihiro, Jumyo, Shinya
• Format: Patent
• Language: English
• Published: 26.04.2022
• Subjects: AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G; APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; GENERAL PROCESSES OF COMPOUNDING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC MACROMOLECULAR COMPOUNDS; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; THEIR PREPARATION OR CHEMICAL WORKING-UP; USE OF INORGANIC OR NON-MACROMOLECULAR ORGANIC SUBSTANCES ASCOMPOUNDING INGREDIENTS; VINEGAR; WINE; WORKING-UP

The present invention relates to a carrier for bio-related molecule immobilization, which comprises a resin substrate, an amino group-containing compound layer that is formed on the resin substrate, and a polyvalent carboxylic acid layer that is formed on the amino group-containing compound layer. The carboxyl groups in the polyvalent carboxylic acid layer are actively esterified; and the (COO peak intensity)/(C-C peak intensity) ratio of the C1s spectrum is from 0.064 to 0.12 (inclusive) if the carrier surface before the active esterification is measured by X-ray photoelectron spectroscopy. The present invention also relates to a carrier for bio-related molecule immobilization, which comprises a water repellent resin substrate, an aminoalkyl silane layer that is formed on the resin substrate and a polyvalent carboxylic acid layer that is formed on the aminoalkyl silane layer, and which is characterized in that: the carboxyl groups in the polyvalent carboxylic acid layer are actively esterified; and the resin substrate is exposed after the formation of the aminoalkyl silane layer.