Iterative genome editing in microbes

The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional countersel...

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Bibliographic Details
Main Authors Patel, Kedar, Blaskowski, Stephen, Meier, Elizabeth Lauren, Netwal, Melissa, Miller, Aaron, Nademanee, Sharon, Coates, Cameron, Szyjka, Shawn, Stonebloom, Solomon Henry, Maxwell, Colin Scott, Cleto, Sara da Luz Areosa, Weyman, Philip
Format Patent
LanguageEnglish
Published 06.07.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.
Bibliography:Application Number: US202016825683