Method for virus clearance

The invention discloses a method for virus clearance of a cell culture medium, comprising the steps of: i) providing a bulk medium portion, comprising amino acids and glucose, and a first additive portion, comprising vitamins in aqueous solution; ii) subjecting the bulk medium portion to a high temp...

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Bibliographic Details
Main Authors Tuohey, Colin R, Castan, Andreas
Format Patent
LanguageEnglish
Published 06.07.2021
Subjects
BEER
BIOCHEMISTRY
CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DISINFECTION, STERILISATION, OR DEODORISATION OF AIR
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES
MEDICAL OR VETERINARY SCIENCE
METALLURGY
METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for virus clearance of a cell culture medium, comprising the steps of: i) providing a bulk medium portion, comprising amino acids and glucose, and a first additive portion, comprising vitamins in aqueous solution; ii) subjecting the bulk medium portion to a high temperature short time treatment (HTST); iii) passing the first additive portion through a virus retentive filter or an ultrafilter; and iv) after steps ii) and iii), mixing the bulk medium portion with the first additive portion to obtain a cell culture medium.
Bibliography:Application Number: US201715718831