Pol6 polymerase variants

The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess...

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Bibliographic Details
Main Authors Boenitz-Dulat, Mara, Thai, Eileen, Amato, Evan, Ayer, Aruna, Schwab, Charles Wayan, Eckert, Barbara, McGaw, Colin, Woersdoerfer, Bigna, Lederman, Ilya, Suko, Shawn, Sarvabhowman, Preethi, Arnold, Cleoma, Wunderlich, David
Format Patent
LanguageEnglish
Published 01.12.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NANOTECHNOLOGY
PERFORMING OPERATIONS
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
SPIRITS
TRANSPORTING
VINEGAR
WINE
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Summary:The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.
Bibliography:Application Number: US201916358580