Massive parallel method for decoding DNA and RNA
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucle...
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Main Authors | , , , |
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Format | Patent |
Language | English |
Published |
02.06.2020
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Subjects | |
Online Access | Get full text |
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Summary: | This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the -OH group at the 3′-position of the deoxyribose. |
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Bibliography: | Application Number: US201514670748 |