Cellulolytic compositions comprising monooxygenase polysaccharide enzymes with improved activity

• Main Authors: Díez García, Bruno, Sánchez Zamorano, Laura, Ledesma García, Laura, Martín Pérez, Lucía, Ramos Martín, Juan Luis, Platero Gómez, Ana Isabel, Valbuena Crespo, Noelia, Bermúdez Alcántara, María de los Angeles, Moreno Pérez, Antonio Javier, Gavaldá Martín, Sandra, Pérez Gómez, Dolores, Álvarez Núñez, Consolación, Reyes Sosa, Francisco Manuel, Rocha Martín, Javier, Viñas De La Cruz, Laura
• Format: Patent
• Language: English
• Published: 21.04.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This improvement is achieved by the addition of a nickel cation to said enzymatic mixtures before and/or during the saccharification step. Thus, the invention provides compositions comprising PMOs, cellulolytic enzymes and a nickel cation, as well as methods for preparing said compositions and methods for producing fermentable sugars and bioproducts, preferably bioethanol, from cellulosic biomass in which said compositions are used.