Collecting apparatus for extracellular vesicles and method for using the same

• Main Authors: Kwak, Rhokyun, Park, Jaesung, Kang, Ji Yoon, Cho, Siwoo
• Format: Patent
• Language: English
• Published: 10.03.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

Provided are an extracellular vesicle collecting apparatus using an electrode and a porous membrane and a method for using the same. The extracellular vesicle collecting apparatus includes i) an upper frame in which a pair of through holes is disposed so that a buffer solution flows therethrough, ii) a buffer tube which is inserted in the through holes and in which the buffer solution flows, iii) a positive electrode disposed on the upper frame, iv) a porous membrane located below the upper frame, v) a spacer which is located below the porous membrane and has a hollow space disposed therein, vi) a plurality of guide tubes through which a blood flows in the hollow space, vii) a lower frame which is coupled to the upper frame to be opposite to the upper frame and receives the porous membrane and the spacer therein, and viii) a negative electrode provided below the lower frame. Extracellular vesicles contained in the blood are collected by the porous membrane.