Method and arrangement for detecting binding events of molecules

• Main Authors: Mannocci, Luca, Wieduwild, Robert, Lin, Weilin, Reddavide, Francesco, Herrmann, Jana, Zhang, Yixin
• Format: Patent
• Language: English
• Published: 07.01.2020
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; DERIVATIVES THEREOF; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; SUGARS; TESTING; VINEGAR; WINE

In a method and arrangement for detecting binding events of molecules, at least one second molecule covalently bound to a ligand, and optionally bonded to a third molecule bound to a ligand, forms a specific non-covalent fond with a first molecule immobilised on a bioactive surface by a covalent bond, and binding events between an analyte molecule and the second-molecule bound ligand are detected by an analytical measuring method, wherein the specific non-covalent bond between the first and second molecules is breakable by supplying a buffer solution, and, hence, a specific ligand, or two, different specific ligands are made available on the bioactive surface in an easily reversible manner.