METHOD TO ISOLATE ZOOSPORANGIA OF THE POTATO CANKER PATHOGEN SYNCHYTRIUM ENDOBIOTICUM (SCHILB) PERC. FROM THE SOIL

A method to isolate zoosporangia of the potato canker pathogen Synchytrium endobioticum (Schilb) Perc. from the soil includes drying the soil, grinding with a rubber pestle, bolting, centrifuging, revealing viable zoosporangia. Bolting the soil is carried out using the sieve with pores of 0.5 mm, 0....

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Bibliographic Details
Main Authors ZELIA AVRELIA HEORHIIVNA, MELNYK PAVLO OLEKSIIOVYCH
Format Patent
LanguageEnglish
Published 15.09.2006
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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Summary:A method to isolate zoosporangia of the potato canker pathogen Synchytrium endobioticum (Schilb) Perc. from the soil includes drying the soil, grinding with a rubber pestle, bolting, centrifuging, revealing viable zoosporangia. Bolting the soil is carried out using the sieve with pores of 0.5 mm, 0.25 mm and 0.03 mm, washing the soil sample by ether above the last sieve (0.03 mm) to solve the organic substances. The centrifuging is carried out in a 48.5% solution of sodium iodide with a specific weight of 1.4 with the simultaneous coloration of the zoosporangia of the disease pathogen with the same solution that allows revealing the viable zoosporangia of the disease pathogen.
Bibliography:Application Number: UA20060001600U