Methods of producing bioconjugates of e. coli o-antigen polysaccharides, compositions thereof, and methods of use thereof

Methods of producing bioconjugates of O-antigen polysaccharides covalently linked to a carrier protein using recombinant host cells are provided. The recombinant host cells used in the methods described herein encode a particular oligosaccharyl transferase enzyme depending on the O-antigen polysacch...

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Main Authors GEURTSEN, JEROEN, FAE, KELLEN CRISTHINA, POOLMAN, JAN THEUNIS, BRAUN, MARTIN EDWARD, KOWARIK, MICHAEL THOMAS, BURGHOUT, PIETER JAN, MALLY, MANUELA, WEERDENBURG, EVELINE MARLEEN, IBARRA YON, PATRICIA, ABBANAT, DARREN ROBERT, GAMBILLARA FONCK, VERONICA, CARRANZA SANDMEIER, MARIA PAULA, KEMMLER, STEFAN JOCHEN
Format Patent
LanguageChinese
English
Published 21.07.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Methods of producing bioconjugates of O-antigen polysaccharides covalently linked to a carrier protein using recombinant host cells are provided. The recombinant host cells used in the methods described herein encode a particular oligosaccharyl transferase enzyme depending on the O-antigen polysaccharide bioconjugate to be produced. The oligosaccharyl transferase enzymes can be PglB oligosaccharyl transferase or variants thereof. Also provided are compositions containing the bioconjugates, and methods of using the bioconjugates and compositions described herein to vaccinate a subject against extra-intestinal pathogenic E. coli. (ExPEC).
Bibliography:Application Number: TW20209109076