Modified transposases for improved insertion sequence bias and increased dna input tolerance

Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.

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Bibliographic Details
Main Authors VAIDYANATHAN, RAMESH, OSOTHPRAROP, TRINA FAYE, GRUNENWALD, HAIYING LI, BURGESS, JOSHUA, HE, MOLLY, KHANNA, ANUPAMA, GLOECKNER, CHRISTIAN, SCHLINGMAN, DANIEL, KUERSTEN, SCOTT, KIA, AMIRALI, BOMATI, ERIN, HASKINS, DARIN
Format Patent
LanguageChinese
English
Published 21.01.2020
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.
Bibliography:Application Number: TW20154112180