Thermostable xylanase, production method and use thereof, and method for producing lignocellulosic glycation product

• Main Authors: INOUE, ATSUSHI, OKAKURA, KAORU, TAMAI, HIROKI, YANAI, HISAAKI, YOKOYAMA, FUMIKAZU, OSABE, MASAMI
• Format: Patent
• Language: Chinese; English
• Published: 21.12.2016
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; APPARATUS THEREFOR; BAKERY PRODUCTS; BAKING; BEER; BIOCHEMISTRY; BLEACHING LEATHER OR FURS; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; EDIBLE DOUGHS; ENZYMOLOGY; FATTY ACIDS THEREFROM; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR; HUMAN NECESSITIES; LAUNDERING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PAPER-MAKING; PRESERVATION THEREOF; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; REGENERATION OF PULPING LIQUORS; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; TEXTILES; TREATMENT OF TEXTILES OR THE LIKE; TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable mutant xylanase and provision of a thermostable mutant xylanase that has substitute amino acid residues, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable mutant xylanase, and a thermostable mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50 °C for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has substitute amino acid residues.