Mutant AOX2 promoter, vector carrying same, transformant, and production of heterologous protein

A mutant AOX2 promoter obtained by at least deleting nucleotides 1-725 of the nucleotide sequence of natural AOX2 promoter and mutating a sequence of natural AOX2 promoter in a manner comprising at least one of the three mutation modes of (1) a region extending upstream from nucleotide 1187 inclusiv...

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Bibliographic Details
Main Authors UNO SYUICHI, HIRAMATSU TAKASHI, OI HIDEYUKI, MIURA MASAMI, CYUGENJI MASAKO
Format Patent
LanguageChinese
English
Published 01.11.1998
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A mutant AOX2 promoter obtained by at least deleting nucleotides 1-725 of the nucleotide sequence of natural AOX2 promoter and mutating a sequence of natural AOX2 promoter in a manner comprising at least one of the three mutation modes of (1) a region extending upstream from nucleotide 1187 inclusive and comprising at least nucleotides 845-960 is deleted, (2) thymine (1274, T) is replaced with cytosine (C), and (3) 19bp oligonucleotides equivalent to nucleotides 1296-1314 are inserted between nucleotide 1295 and nucleotide 1296.
Bibliography:Application Number: TW199382108332