Identification system of circulating biomarkers for cancer detection, development method of circulating biomarkers for cancer detection, cancer detection method and kit

• Main Authors: LI, JIAN-HAO, CHEN, POANG, JIANG, PEI-SHIN, HSIEH, HUIU, LIN, CHIH-LUNG
• Format: Patent
• Language: Chinese; English
• Published: 01.07.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

An identification system of circulating biomarkers for cancer detection, a development method of circulating biomarkers for cancer detection, a cancer detection method and a kit are provided, and the development method includes the following steps. Expression levels of multiple genes in normal tissue samples and tumor tissue samples are identified, and genes with high expression levels in tumor tissue samples are selected. Afterwards, a weight of each tissue's contribution to plasma exosomes is calculated using Tissue-specific genes and Group-enriched genes. Next, expression levels of plasma exosome genes of healthy people and cancer patients are compared by the overlapping index, and circulating biomarkers and combinations thereof suitable for detection and evaluation of plasma exosomes are selected.