Lentivirus packaging system and the method of using the same for improving lentivirus production in a host cell

The present invention provides a lentivirus packaging system, which comprises: a transfer plasmid comprising a nucleic acid sequence encoding TAR-reserved- chimeric 5' long terminal repeat (LTR); at least one packaging plasmid comprising a nucleotide sequence encoding TAR RNA binding protein ge...

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Bibliographic Details
Main Authors CHOU, SSU-YU, YANG, YAONG, LIN, CHEN-LUNG, LIN, CHIEN-TING, LIN, WEII
Format Patent
LanguageChinese
English
Published 01.11.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides a lentivirus packaging system, which comprises: a transfer plasmid comprising a nucleic acid sequence encoding TAR-reserved- chimeric 5' long terminal repeat (LTR); at least one packaging plasmid comprising a nucleotide sequence encoding TAR RNA binding protein gene, a nucleotide e sequence of rev gene, a nucleotide sequence of gag gene, and a nucleotide sequence of pol gene; and an envelope plasmid. Due to the expression of TAR RNA binding protein gene by the plasmids, the produced lentivirus have higher virus titer and can improve the transduction rate and the gene delivery efficiency during cell transduction. The present invention further provides a method of improving lentivirus production in a host cell, which comprises using the aforementioned lentivirus packaging system to transfect the host cell. The method can further reduce the cost of making a gene-modified cell.
Bibliography:Application Number: TW202211107832