Production method of recombinant insulin-like growth factor I including a rIGF-1 obtaining step, a SUMO-IGF-1 treatment step, and an eluent obtaining step

The present invention provides a method of producing recombinant insulin-like growth factor I (rIGF-1) as an improved method for obtaining rIGF-1 in which misfolding recombinant insulin-like growth factor I (rIGF-1) is correctly folded with high purity. The production method of I(rIGF-1) comprises:...

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Bibliographic Details
Main Authors TAKASU, MASAHIRO, KAWASHIMA, HIROYUKI, HSUEH, KUANG-LUNG
Format Patent
LanguageChinese
English
Published 16.05.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention provides a method of producing recombinant insulin-like growth factor I (rIGF-1) as an improved method for obtaining rIGF-1 in which misfolding recombinant insulin-like growth factor I (rIGF-1) is correctly folded with high purity. The production method of I(rIGF-1) comprises: (1) a step of obtaining rIGF-1 as a fusion protein (SUMO-IGF-1) with the ubiquitin-like protein SUMO (small ubiquitin-related modifier); (2) a step of treating SUMO-IGF-1 by SUMO protease; and (3) a step of applying the treatment solution of step (2) to cation exchange chromatography, and obtaining an eluent containing rIGF-1 by gradient elution.
Bibliography:Application Number: TW20209138611