Method of producing 2,3-butanediol with high yield

• Main Authors: MA, TIEN-YANG, LIANG, KE-MING, SHEN, ROA-PU, GUO, GIA-LUEN, HUANG, CHIUNG-FANG
• Format: Patent
• Language: Chinese; English
• Published: 16.10.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

A method is provided for producing 2,3-butanediol. An escherichia coli (E. coli) strain obtained through an innovation of genetic modification is used. In vivo of the strain, desired genes for producing 2,3-butanediol are expressed through gene transfer in a gene-recombination way. The present invention is characterized in that, during producing 2,3-butanediol, the strain does not change its yield owing to the change of oxygen concentration in the fermentation environment. The strain is capable of self-regulation by using an in-vivo metabolic pathway for dealing with the external environmental oxygen concentration. Thus, 2,3-butanediol is continuously produced with a high yield.